2′-pRib-AMP

2′-pRib-AMP is the monomeric metabolite of poly(ADP-ribose) (PAR) and can be used to detect and analyse PAR-specific binding properties of poly(ADP-ribose) polymerases. Recent studies have shown that plant nucleotide-binding leucine-rich repeat (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain recognise pathogen effectors. The TIR-enabled nicotinamideadenine dinucleotide hydrolase (NADase) activity is used to mediate the immune response. It has been shown that TIR-containing proteins can biocatalyse the production of 2′-pRib-AMP from NAD+ in plants, and it has been postulated that 2′-pRib-AMP may have a second messenger function in TIR signalling of plant immunity.