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2-(4-Dehydroxy)coelenterazine

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Chemiluminescence probe of the superoxide anion and peroxynitrite

Derivative of coelenterazine (Prod. No. ENZ-52054) with ~20 times higher luminescence intensity.

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Alternative Name	Coelenterazine h, Luciferin / Renilla
Appearance	Yellow to orange solid.
CAS	50909-86-9
Formula	C₂₆H₂₁N₃O₂
MW	407.5
Purity	≥95%
Solubility	Soluble in 100% ethanol or methanol; slightly soluble in water. Avoid DMSO as product is unstable in this solvent.

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Protect from light. Keep under inert gas. Keep calcium free when stored in solution.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Importance of lipid-exposed residues in transmembrane segment four for family B calcitonin receptor homo-dimerization: K.G. Harikumar, et al.; Regul. Pept. 164, 113 (2010), Abstract
Functional importance of a structurally distinct homodimeric complex of the family B G protein-coupled secretin receptor: F. Gao, et al.; Mol. Pharmacol. 76, 2640 (2009), Abstract — Full Text
Intracellular Ca2+ signals in Dictyostelium chemotaxis are mediated exclusively by Ca2+ influx: T. Nebl & P.R. Fisher; J. Cell Sci. 110, 2845 (1997), Abstract
Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins: M.R. Knight, et al.; J. Cell. Biol. 121, 83 (1993), Abstract
Preparation and handling of aequorin solutions for the measurement of cellular Ca2+: O. Shimomura; Cell Calcium 12, 635 (1991), Abstract
Semi-synthetic aequorins with improved sensitivity to Ca2+ ions: O. Shimomura, et al.; Biochem. J. 261, 913 (1989), Abstract

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