2′-/3′-Fluo-AHC-5′-AMP

2’-/3’-Fluo-AHC-5’-AMP is an analogue of 5’-AMP in which 5-carboxyfluorescein has been attached either to the ribose 2’-hydroxy group or to the 3’-hydroxy group via an aminohexylcarbamoyl spacer. 2’-/3’-Fluo-AHC-5’-AMP is a fluorescent analogue of 5’-AMP (λ_exc 494 nm, λ_em 517 nm) and can be used as a calibrator in phosphodiesterase (PDE) assays using 2’-Fluo-AHC-cAMP (FAM-cAMP, Prod. No. BLG-F003) as a substrate.