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17β-Estradiol high sensitivity ELISA kit

ADI-900-174

78 citations

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Highly sensitive ELISA, detecting as little as 14 pg/ml of 17β-Estradiol

Highly sensitive measurement, as little as 14 pg/ml
Fast and reliable results from up to 37 samples in duplicate in just 3 hours
Fully quantitative results that surpass semi-quantitative Western blot analysis
Convenient pre-coated plate and color-coded reagents reduces errors

The 17β-Estradiol (serum/plasma) EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours.

Biologically active estrogen, 17β-estradiol, is an important sex hormone, but also makes an important contribution to the high concentrations of estrogens, which are present in malignant breast tissues.

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Product Details

Alternative Name	Estrogen
Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of 17β-Estradiol in plasma and serum from any species.
Assay Time	3 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Crossreactivity	17β-Estradiol (100%), Estrone (17.8%), Estriol (0.9%), DHT (0.12%), Ethynylestradiol-7α (0.043%), Dehydroisoandroesterone (0.38%), Estradiol-17α (0.032%), Testosterone (0.01%), Progesterone (<0.001%), Danazol (<0.001%)
Sensitivity	14.0 pg/ml (range 15.6 – 1,000 pg/ml)
Species Reactivity	Species independent
Technical Info / Product Notes	Cited samples: Wildlife Endocrinology: Cited Sample Types
Wavelength	405 nm

Handling & Storage

Use/Stability	The 17β-Estradiol Antibody and Conjugate are stable at -20°C. All other components of this kit are stable at +4°C until the kit’s expiration date. The Antibody and Conjugate should be stored at -20°C upon receipt.
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Serum 17β-estradiol inversely correlates with circulating group 2 innate lymphoid cells in a cohort of asthmatic patients: Myers, E. J., Aamodt, S. E., et al.; Front. Immunol. 16, 1555228 (2025), Abstract
Differential Effects of Canonical Androgens and 11-Ketotestosterone on Reproductive Phenotypes and Folliculogenesis in Mouse Model of PCOS: Tsai, Y. R., Liao, Y. N., et al.; Biomedicines 13, 1077 (2025), Abstract
Acute stimulation of PBMCs drives switch from dopamine-induced anti- to proinflammatory phenotype of monocytes only in women: Fleige, L., Capellino, S., et al.; Biol. Sex Differ. 16, 8 (2025), Abstract
Impact of Dietary Isoflavones in Standard Chow on Reproductive Development in Juvenile and Adult Female Mice with Different Metabolic Phenotypes: Meyer, Z., Soukup, S. T., et al.; Nutrients 16, (2024), Abstract
17 b ethinylestradiol modulates endothelial function in ovariectomized rat carotid arteries: F. Yuan, et al.; Eur. J. Pharmacol. 944, 175525 (2023), Abstract
Estrogen and progesterone receptors and antioxidant enzymes are expressed differently in the uterus of domestic cats during the estrous cycle: A.E. de Jesus Nascimento, et al.; Theriogenology 203, 1 (2023), Abstract
Steroid receptor coactivator-2 drives epithelial reprogramming that enables murine embryo implantation: Maurya, V. K., Szwarc, M. M., et al.; FASEB J. 37, e23313 (2023), Abstract
Rat Ovarian Function Is Impaired during Experimental Autoimmune Encephalomyelitis: Milosevic, A., Lavrnja, I., et al.; Cells 12, (2023), Abstract
Perfluorooctane sulfonic acid modulates expression of placental steroidogenesis-associated genes and hormone levels in pregnant rats: S.V. Dangudubiyyam, et al.; Reprod. Toxicol. 118, 108390 (2023), Application(s): Rat plasma, Abstract
HERC1 deficiency causes osteopenia through transcriptional program dysregulation during bone remodeling: Pedrazza, L., Martinez-Martinez, A., et al.; Cell Death Dis. 14, 17 (2023), Abstract