15-deoxy-Δ^12,14-PGJ₂ ELISA kit

• High sensitivity, measuring as low as 36.8 pg/ml
• Fast results, much less time consuming than GC/MS analysis
• Simple and ready-to-use liquid color-coded reagents reduce error
• Fully quantitative results that surpass semi-quantitative Western blot analysis

The 15-deoxy-Δ^12,14-PGJ₂ ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. This kit saves time and is much less time consuming than GC/MS analysis. Ready-to-use liquid color-coded reagents reduce laboratory error.

15-deoxy-Δ^12,14-Prostaglandin J₂ (15-d-PGJ₂) is one of the ultimate dehydration products of PGD₂, formed via the intermediate Δ¹²-PGJ₂. 15-d-PGJ₂ is physiologically present in body fluids at concentration of 10^-12 to 10^-9 M, but increases dramatically under stress such as infection and inflammation. 15-d-PGJ₂ exerts both pro- and anti-inflammatory effects in a cell-type and concentration-dependent manner, with many of these effects mediated via binding to the γ isoform of the peroxisome proliferators-activated receptor (PPAR-γ). 15-d-PGJ₂ appears to induce HO-1 expression by a PPAR-γ-independent mechanism mediated by the generation of reactive oxygen species (ROS).