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This antibody is covered by our Worry-Free Guarantee.

Flow cytometric analysis of Human B2M / Beta-2-microglobulin expression on human whole blood lymphocytes. Cells were stained with purified anti-Human B2M / Beta-2-microglobulin, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

Immunofluorescence staining of B2M in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human B2M monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.

Immunochemical staining of human B2M in human ovarian cancer with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.

Immunochemical staining of human B2M in human skin with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.

Anti-B2M mouse monoclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: U937 Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:14 kDa

Anti-Beta-2 microglobulin mouse monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Beta-2 microglobulin knockout Hela Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:14 kDa.






Product Details
Alternative Name |
B2M |
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Application |
ELISA, Flow Cytometry, ICC, IF, IHC (PS), WB |
Clone |
34 |
Formulation |
Liquid. 0.2 μm filtered solution in PBS. Preservative free. |
Host |
Mouse |
Immunogen |
Recombinant human Beta-2 microglobulin/B2M protein. |
Isotype |
IgG1 |
Purity Detail |
Protein A purified. |
Recommendation Dilutions/Conditions |
Western Blot (1:500-1:1’000)ELISA (1:1’000-1:2’000)Immunohistochemistry (paraffin sections) (1:30-1:100)Immunocytochemistry (1:20-1:100)Immunofluorescence (1:20-1:100)Flow Cytometry (1:25-1:100)ELISA (Detection)(1:1’000-1:10’000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
Species Reactivity |
Human |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Handling |
Protected from light. Avoid freeze-thaw cycles. |
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Long Term Storage |
-20°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Certificate of Analysis
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SDS
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