AMPIVIEW® RNA probes
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This antibody is covered by our Worry-Free Guarantee.
Product Details
| Application |
ICC, IP, WB |
|---|---|
| Clone |
15D9 |
| Crossreactivity |
Does not cross-react with β-catenins. |
| Formulation |
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose. |
| Host |
Mouse |
| Immunogen |
Recombinant human α-catenin. |
| Isotype |
IgG1 |
| Positive Control |
Included. (Prod. No. ALX-840-026) |
| Purity Detail |
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography. |
| Recommendation Dilutions/Conditions |
Immunocytochemistry (0.1-1µg/ml; formaldehyde fixation is tolerated)Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Reconstitution |
Reconstitute with 1ml distilled water (15 min., RT). |
| Species Reactivity |
Dog, Human, Mouse, Rat |
| UniProt ID |
P35221 (α1), P26232 (α2), Q9UI47 (α3) |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Use/Stability |
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months. |
|---|---|
| Handling |
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- α-catenin phosphorylation is elevated during mitosis to resist apical rounding and epithelial barrier leak: Le, P. M., Quinn, J., et al.; Biol. Open 14, (2025), Application(s): Western blot, Abstract
- Vinculin Y822 phosphorylation regulates cardiomyocyte adhesion dynamics and adherens junction maturation in the heart: Li, X., Wortelboer, R., et al.; bioRxiv , (2024), Reactant(s): Mouse
- Cell division-dependent dissemination following E-cadherin loss underlies initiation of diffuse-type gastric cancer: Monster, J. L., Kemp, L. J., et al.; J. Pathol. 263, 226 (2024), Abstract
- A mechanical G2 checkpoint controls epithelial cell division through E-cadherin-mediated regulation of Wee1-Cdk1: Donker, L., Houtekamer, R., et al.; Cell Rep. 41, 111475 (2022), Abstract
- An asymmetric junctional mechanoresponse coordinates mitotic rounding with epithelial integrity: Monster, J. L., Donker, L., et al.; J. Cell Biol. 220, (2021), Abstract
- Adherens junction regulates cryptic lamellipodia formation for epithelial cell migration.: Takeichi, M., Mimori-Kiyosue, Y., et al.; J. Cell Biol. 219, (2020), Application(s): IF / Reactant(s): Human, Abstract
- Adherens junction serves to generate cryptic lamellipodia required for collective migration of epithelial cells: Takeichi, M., Mimori-Kiyosue, Y., et al.; bioRxiv , (2020), Application(s): IF
- Vinculin anchors contractile actin to the cardiomyocyte adherens junction: Merkel, C. D., Li, Y., et al.; Mol. Biol. Cell 30, 2639 (2019), Abstract
- The N-cadherin interactome in primary cardiomyocytes as defined using quantitative proximity proteomics.: Li, Y., Merkel, C. D., et al.; J. Cell Sci. 132, (2019), Application(s): ICC-IF / Reactant(s): Mouse, Abstract
- The N-cadherin interactome in primary cardiomyocytes as defined by quantitative proximity proteomics: Li, Y., Merkel, C. D., et al.; bioRxiv , (2018)
- αE-catenin is a candidate tumor suppressor for the development of E-cadherin-expressing lobular-type breast cancer.: de Groot, J. S., Ratze, M. A., et al.; J. Pathol. 245, 456 (2018), Application(s): ICC-IF, IF, Abstract
- Girdin/GIV regulates collective cancer cell migration by controlling cell adhesion and cytoskeletal organization.: Wang, X., Enomoto, A., et al.; Cancer Sci. 109, 3643 (2018), Application(s): WB, Abstract
- A mechanically active heterotypic E-cadherin/N-cadherin adhesion enables fibroblasts to drive cancer cell invasion.: Sahai, E., Weston, A., et al.; Nat. Cell Biol. 19, 224 (2017), Application(s): IHC / Reactant(s): Human, Abstract
- Catenins Steer Cell Migration via Stabilization of Front-Rear Polarity: Vassilev, V., Platek, A., et al.; Dev. Cell 43, 463 (2017), Abstract
- Biochemical analysis of force-sensitive responses using a large-scale cell stretch device: Renner, D. J., Ewald, M. L., et al.; Cell. Adh. Migr. 11, 504 (2017), Abstract
- A simple elution strategy for biotinylated proteins bound to streptavidin conjugated beads using excess biotin and heat: J. S. Cheah, et al.; Biochem. Biophys. Res. Commun. 493, 1522 (2017), Application(s): Western Blot on MDCK cells, Abstract
- Adhesion to the host cell surface is sufficient to mediate Listeria monocytogenes entry into epithelial cells.: Lauer, P., Gloerich, M., et al.; Mol. Biol. Cell 28, 2945 (2017), Application(s): WB / Reactant(s): Dog, Abstract
- Control of cell-cell forces and collective cell dynamics by the intercellular adhesome: E. Bazellières, et al.; Nat. Cell Biol. 17, 409 (2016), Reactant(s) Human, Abstract
- Force dependent biotinylation of myosin IIA by α-catenin tagged with a promiscuous biotin ligase.: Ueda, S., Yamada, S., et al.; PLoS One 10, e0122886 (2015), Application(s): WB / Reactant(s): Dog, Abstract
- Twist1-induced dissemination preserves epithelial identity and requires E-cadherin.: Ewald, A. J., Bader, J. S., et al.; J. Cell Biol. 204, 839 (2014), Application(s): IF, IHC-IF, WB / Reactant(s): Mouse, Abstract
- N-cadherin dependent collective cell invasion of prostate cancer cells is regulated by the N-terminus of α-catenin: Cui, Y., Yamada, S., et al.; PLoS One 8, e55069 (2013), Abstract
- αE-catenin actin-binding domain alters actin filament conformation and regulates binding of nucleation and disassembly factors: Hansen, S. D., Kwiatkowski, A. V., et al.; Mol. Biol. Cell 24, 3710 (2013), Abstract
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