Home Shop αB-Crystallin monoclonal antibody (1B6.1-3G4)

αB-Crystallin monoclonal antibody (1B6.1-3G4)

ADI-SPA-222

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Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.

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This antibody is covered by our Worry-Free Guarantee.

αB-Crystallin monoclonal antibody (1B6.1-3G4) Immunohistochemistry — Immunohistochemistry analysis of human Heart tissue stained with alpha B-Crystallin, mAb (1B6.1-3G4) at 10µg/ml.

αB-Crystallin monoclonal antibody (1B6.1-3G4) Western blot — Western Blot analysis of αB-Crystallin mAb (1B6.1-3G4) (Prod. No. ADI-SPA-222): Lane 1: MW Marker, Lane 2: α-Crystallin (bovine), (native) (Prod. No. ADI-SPP-225), Lane 3: αB-Crystallin (bovine), (native) (Prod. No. ADI-SPP-227), Lane 4: αA-Crystallin (bovine), (native) (Prod. No. ADI-SPP-226), Lane 5: β-Crystallin (bovine), (native) (Prod. No. ENZ-PRT224).

Product Details

Alternative Name	CRYAB, HSPB5
Application	ICC, IF, IHC (PS), WB
Application Notes	Detects a band of ~20kDa by Western blot.
Clone	1B6.1-3G4
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Host	Mouse
Immunogen	Bovine αB-crystallin.
Isotype	IgG1
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Immunohistochemistry (paraffin sections, 10µg/ml)Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from ascites.
Species Reactivity	Bovine, Chicken, Human, Mouse, Rat
UniProt ID	P02510
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Skeletal Muscle Heat Shock Protein Content and the Repeated Bout Effect.: Locke, M., Bruccoleri, G., et al.; Int. J. Mol. Sci. 25, (2024), Reactant(s): Rat, Abstract
The small heat shock protein αB-Crystallin protects versus withaferin A-induced apoptosis and confers a more metastatic phenotype in cisplatin-resistant ovarian cancer cells: M.M. Carmichael, et al.; PLoS One 18, e0281009 (2023), Abstract
Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1: N. Shiba, et al.; Mol. Ther. Nucleic Acids 34, 102060 (2023), Application(s): WB / Reactant(s) Human, Abstract
Generation of a homozygous CRYAB p.Arg120Gly mutant (UKEi001-A-1) from a human iPSC line.: Pietsch, N., Cheng, J., et al.; Stem Cell Res. 71, 103188 (2023), Reactant(s): Human, Abstract
Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin.: Tanaka, Y., Kadota, S., et al.; Stem Cell Res. Ther. 14, 240 (2023), Application(s): WB / Reactant(s): Human, Abstract
Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis: Z. Li, et al.; Int. J. Biol. Sci. 18, 2018 (2022), Application(s): IHC, WB / Reactant(s) Mouse, Abstract
Molecular Features of Lymph Node Metastasis in T1/2 Colorectal Cancer from Formalin-Fixed Paraffin-Embedded Archival Specimens: P. Steffen, et al.; J. Proteome Res. 20, 1304 (2021), Abstract
Loss of FYCO1 leads to cataract formation: S. Kiyotoshi, et al.; Sci. Rep. 11, 13771 (2021), Abstract
αB-crystallin response to a pro-oxidant non-cytotoxic environment in murine cardiac cells: An in vitro” and “”in vivo”” study”””: A. Antonioni, et al.; Free Radic. Biol. Med. 152, 301 (2020), Abstract
Coordinated alpha-crystallin B phosphorylation and desmin expression indicate adaptation and deadaptation to resistance exercise-induced loading in human skeletal muscle.: Höhfeld, J., Bloch, W., et al.; Am. J. Physiol. Cell Physiol. 319, C300 (2020), Reactant(s): Human, Abstract