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Tissue Analysis
How can slides be stored after ISH?
That depends mostly on fixation/embedding method and the type of ISH performed. After chromogenic ISH on FFPE or cryopreserved tissue,…
Tue, Jul 18, 2023
How big are ISH probes?
There is no defined answer. Depending on the tissue specimen, target type, and sequence, ISH probes might vary from below…
Tue, Jul 18, 2023
Probe Design and Generation – How are DNA probes prepared?
First choice is to explore if commercially ready-to-use probes are available for specific targets. Enzo Life Sciences offers a selection…
Tue, Jul 18, 2023
How can RNA degradation in the sample be prevented?
When RNA is the target of ISH, preservation of RNA in the sample is very important given the ubiquitous presence…
Tue, Jul 18, 2023
Is there a specifc type of glass slide recommended for ISH?
Positively charged glass slides have proven optimal to draw tissue sample sections for subsequent ISH on. Alternatively, poly-L-lysine or silanized…
Tue, Jul 18, 2023
Tissue specimen preparation – How should samples be stored and for how long?
While whole tissue specimen are sometimes used for long-term storage, most often glass slide-mounted tissue sections are stored at 4°C.…
Tue, Jul 18, 2023
What is the recommended thickness of the section to perform ISH?
Ideal section thickness depends on fixation and embedding method, as well as microscope that is available. FFPE tissue is ideally…
Tue, Jul 18, 2023
Is BIO-PROBE® 3’-Oligonucleotide labeling kit with bio-16-ddUTP, ENZ-42730-31 compatible with EMSA?
BIOPROBE® 3′-Oligonucleotide labeling kit with bio-16-ddUTP, ENZ-42730-31can be used for gel shift assays since the reaction yields uniformly labeled product.
Tue, Jul 18, 2023
Can I detect constitutive HSP70 with ADI-EKS-700B or ADI-EKS-715?
These assays are specific for induced HSP70 and will not detect constitutive HSP70. Taking this into account, you need to…
Tue, Jul 18, 2023
How much sample do I need to use and what are the suggested dilutions when using ADI-EKS-700B, ADI-EKS-715, ADI-EKS-725A, ADI-EKS-750, and ENZ-KIT-101?
The amount of sample required and the dilutions needed for your assay depend upon the amount of HSP70, HSP70B, or…
Tue, Jul 18, 2023