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Can I detect cytoplasmic cAMP/cGMP with these Complete ELISA kits?
These are colorimetric ELISA kits for the quantification of both intra- and extra-cellular cAMP/cGMP in cell lysates, culture supernatants, serum,…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP complete ELISA kit be used with the cGMP complete ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the…
Tue, Jul 18, 2023
EGS (ethyleneglycol-bis-succinimidyl-succinate) Fixation
EGS (ethyleneglycol-bis-succinimidyl-succinate) fixation (method to preserve microtubules and membrane bound antigens) Rinse cells with pre-warmed (37°C) PBS. Prepare EGS stock…
Tue, Jul 18, 2023
Formaldehyde Fixation
Formaldehyde fixation (for membrane associated components) Fixative Preparation Dissolve 16 g paraformaldehyde in 80 ml dH2O by stirring at 70°C…
Tue, Jul 18, 2023
Immunohistochemistry Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Membrane Preparation Protocol
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Membranes ELISA Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Blocking Peptide Neutralization
Reconstitute the lyophilized peptide with 0.1 mL distilled water or PBS. Be careful to reconstitute entire contents of vial as…
Tue, Jul 18, 2023
Immunoprecipitation of Phosphoserine-, Phosphothreonine- and Phosphotyrosine-Containing Proteins
Important Remarks: Rinse all plastic vessels with BSA solution (10 mg/mL in lysis buffer). Use lipid-free BSA, e.g., Sigma A7906. For…
Tue, Jul 18, 2023
Western Blot Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR polyclonal antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023