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Can I detect cytoplasmic cAMP/cGMP with these Direct ELISA kits?
These are colorimetric ELISA kits developed specifically for the measurement of intracellular cAMP/cGMP in cell or tissue lysates from any…
Tue, Jul 18, 2023
Is it possible to detect autophagy by fluorescence microscopy using 3D culture systems?
We have not tested the dye in 3D culture systems, but the small cell clusters are probably dye-accessible. For live…
Tue, Jul 18, 2023
EGS (ethyleneglycol-bis-succinimidyl-succinate) Fixation
EGS (ethyleneglycol-bis-succinimidyl-succinate) fixation (method to preserve microtubules and membrane bound antigens) Rinse cells with pre-warmed (37°C) PBS. Prepare EGS stock…
Tue, Jul 18, 2023
Formaldehyde Fixation
Formaldehyde fixation (for membrane associated components) Fixative Preparation Dissolve 16 g paraformaldehyde in 80 ml dH2O by stirring at 70°C…
Tue, Jul 18, 2023
Methanol Fixation
Methanol fixation (for cytoskeletal components) The methanol fixation is an easy method. However, it frequently solubilizes and removes membrane bound…
Tue, Jul 18, 2023
Checking for Interference or Linearity
Interference is when some component of a sample prevents the appropriate binding events to take place. Percent recovery is the…
Tue, Jul 18, 2023
Competitive ELISA Assay
For small molecule analytes, typically less than 10 kDa, the competitive ELISA is used. An advantage of this assay over…
Tue, Jul 18, 2023
Immunometric ELISA
One of the most useful of the immunoassays is the two antibody immunometric or “sandwich” ELISA. This assay is used…
Tue, Jul 18, 2023
Immunohistochemistry Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Membrane Preparation Protocol
Note: The following protocol has been developed for use with GPCR Polyclonal Antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023