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Why are the Gold 525 dUTPs bleeding through in the red channel?
If an epifluorescence microscope is used for visualization, the incorporated dUTPs should be matched to the filters equipped in the…
Tue, Jul 18, 2023
Why should post-hybridization washes be carried out?
Post-hybridization washing after ISH will reduce non-specific signal by removing probes that are lightly bound to off-target sequences. Most frequently,…
Tue, Jul 18, 2023
How can slides be stored after ISH?
That depends mostly on fixation/embedding method and the type of ISH performed. After chromogenic ISH on FFPE or cryopreserved tissue,…
Tue, Jul 18, 2023
How big are ISH probes?
There is no defined answer. Depending on the tissue specimen, target type, and sequence, ISH probes might vary from below…
Tue, Jul 18, 2023
Probe Design and Generation – How are DNA probes prepared?
First choice is to explore if commercially ready-to-use probes are available for specific targets. Enzo Life Sciences offers a selection…
Tue, Jul 18, 2023
How can RNA degradation in the sample be prevented?
When RNA is the target of ISH, preservation of RNA in the sample is very important given the ubiquitous presence…
Tue, Jul 18, 2023
Is there a specifc type of glass slide recommended for ISH?
Positively charged glass slides have proven optimal to draw tissue sample sections for subsequent ISH on. Alternatively, poly-L-lysine or silanized…
Tue, Jul 18, 2023
Guidelines For Handling Small Molecules
These guidelines are general tips for use with handling small molecules. Small molecules require careful handling procedures to preserve their…
Tue, Jul 18, 2023
Guidelines For Handling Proteins
These guidelines are general tips for use with handling proteins. Proteins require careful handling procedures to preserve protein function, stability,…
Tue, Jul 18, 2023
How do I obtain the Certificate of Analysis for Enzo products?
The Certificate of Analysis are available for download directly from the product page. Please click on the Certificate of Analysis…
Tue, Jul 18, 2023