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Life Sciences Products
Is protein blocking really necessary in ISH?
Blocking with bovine serum albumin (BSA), milk or serum is always recommended when ISH signals are detected by antibodies (e.g.…
Tue, Jul 18, 2023
Why are the Gold 525 dUTPs bleeding through in the red channel?
If an epifluorescence microscope is used for visualization, the incorporated dUTPs should be matched to the filters equipped in the…
Tue, Jul 18, 2023
How can slides be stored after ISH?
That depends mostly on fixation/embedding method and the type of ISH performed. After chromogenic ISH on FFPE or cryopreserved tissue,…
Tue, Jul 18, 2023
Can multiple targets be detected with ISH?
Yes, it is common practice to stain with multiple ISH probes in parallel. However, while continuously-labeled immunofluorescent probes can be…
Tue, Jul 18, 2023
Probe Design and Generation – How are DNA probes prepared?
First choice is to explore if commercially ready-to-use probes are available for specific targets. Enzo Life Sciences offers a selection…
Tue, Jul 18, 2023
Is there a specifc type of glass slide recommended for ISH?
Positively charged glass slides have proven optimal to draw tissue sample sections for subsequent ISH on. Alternatively, poly-L-lysine or silanized…
Tue, Jul 18, 2023
Guidelines For Handling Small Molecules
These guidelines are general tips for use with handling small molecules. Small molecules require careful handling procedures to preserve their…
Tue, Jul 18, 2023
Guidelines for For Handling Antibodies
Antibodies require careful handling procedures to preserve antibody function, stability, and integrity. Please refer to the data sheet(s), product webpage,…
Tue, Jul 18, 2023
Tissue specimen preparation – How should samples be stored and for how long?
While whole tissue specimen are sometimes used for long-term storage, most often glass slide-mounted tissue sections are stored at 4°C.…
Tue, Jul 18, 2023
Why your SCREEN-WELL® Natural product library (BML-2865) supplied in mg/mL instead of mM?
When our SCREEN-WELL® Natural product library (BML-2865) library was originally developed, we included some very exotic compounds. Unlike synthetic chemicals,…
Tue, Jul 18, 2023