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Nucleic Acid Labeling
What is the purity, volume and amounts of Bio-17-ATP, ENZ-42817 provided?
Biotin-17-adenosine-5′-triphosphate is =93% (HPLC), the amount is 250 nmol in a volume of 25 ul.
Tue, Jul 18, 2023
What is the suggested volume of SEEBRIGHT® Red 598 dUTP, ENZ-42855 and SEEBRIGHT® Green 496 dUTP, ENZ-42831 with Nick translation DNA labeling system 2.0, ENZ-GEN111-0050?
We suggest to reconstitute the dyes (0.3 mM) and use the same volumes as mentioned in the manual of the…
Tue, Jul 18, 2023
What is the recommendation to reconstitute the SEEBRIGHT® lyophilized dUTPs, ENZ-42853L, ENZ-42843L, ENZ-42831L, ENZ-42842L?
Dissolving in 10 mM TE pH8, is the preferred method since the nucleotides are stable for long term -20°C storage.…
Tue, Jul 18, 2023
Does BIOARRAY™ Single-round RNA amplification and biotin labeling system, ENZ-42420 and ENZ-42421 have all the items necessary for amplification and labeling?
This kit, provided in 2 sizes (10 reactions: ENZ-42420; 100 reactions: ENZ-42421) contains all the buffers, enzymes, purification columns, elution…
Tue, Jul 18, 2023
Can gene-specific primers be used for cDNA synthesis with BIOARRAY™ Single-round RNA amplification and biotin labeling system, ENZ-42420 and ENZ-42421?
RNA amplification with BIOARRAYTM Single-round RNA amplification and biotin labeling system requires the use of primers included in the kit.…
Tue, Jul 18, 2023
What are the spectra of the different fluorophore-labelled dUTPs?
The individual spectra of each SEEBRIGHT® fluorescent dUTP can additionally be found on the individual product's webpage. Excitation and emission…
Tue, Jul 18, 2023
Which dUTPs are best combined for multiplexing?
If probes are selected so that both location and color contribute to identification, it is theoretically possible to combine all…
Tue, Jul 18, 2023
Can the dUTPs be used for TUNEL assays or only for ISH probe labeling?
The labeled dUTPs can be used for a variety of applications besides in situ hybridization. They can be used as…
Tue, Jul 18, 2023
What is the recommended ratio of dTTP to fluorophore-dUTP when using the Nick translation DNA labeling system 2.0?
The SEEBRIGHT® fluorophore-labeled dUTPs are supplied as a 1 mM solution. We recommend to prepare working concentration of 0.3 mM…
Tue, Jul 18, 2023
What are the correction factors for measuring dye incorporation in a NanoDrop?
The correction factors for 260/280nm detection are as follows: Correction Factor at Aqua 431 Green 496 Green 500 Gold 525 Gold 500 Orange…
Tue, Jul 18, 2023