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Multidrug Resistance
How does EFLUXX-ID® Green multidrug resistance assay kit, ENZ-51029 and EFLUXX-ID® Gold multidrug resistance assay kit, ENZ-51030 detect activity of transporter proteins?
EFLUXX-ID® Green and Gold Dyes in ENZ-51029 and ENZ-51030 kits are proprietary AM-esters. EFLUXX-ID® dye is a hydrophobic non-fluorescent compound…
Tue, Jul 18, 2023
Do the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) require a reading by FACS or could a fluorescence reader such as Envision reader be used?
The the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for Flow Cytometry and Microscopy. This does not…
Tue, Jul 18, 2023
Have the eFluxx-ID® Green multidrug resistance assay kit, ENZ-51029 and eFluxx-ID® Gold multidrug resistance assay kit, ENZ-51030 been tested and used in clinical trial samples such as PBMC, bone marrow samples?
We have not used EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) with clinical samples and we are not aware if…
Tue, Jul 18, 2023
Does the eFluxx-ID® Green multidrug resistance assay kit, ENZ-51029 and eFluxx-ID® Gold multidrug resistance assay kit, ENZ-51030 kits work with frozen samples?
The EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for live cell analysis. If cells are healthy upon…
Tue, Jul 18, 2023
Can a multiplex assay be set up for testing three pumps at a time with The EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030)?
It is not possible to differentiate the pumps in a single assay with the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029,…
Tue, Jul 18, 2023
Can different colored dyes be used to measure a different parameter with eThe eFluxx-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030)?
It is possible to use a dye which would not have spectral overlap with the EFLUXX-ID® dyes (ENZ-51029, ENZ-51030) and…
Tue, Jul 18, 2023
What is the reason for the use of dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020)? Should this be used with all cell lines or is there an exception?
The use of the dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020) can help preventing the…
Tue, Jul 18, 2023
Cells do not exhibit fluorescence after incubation with the detection reagent.
Cell viability is low. Cells should be in log growth phase. Cell samples cannot be kept longer than 6 hours…
Tue, Jul 18, 2023
There are differences in MFI (mean fluorescence intensity) values between cell lines.
Detection reagent accumulation may be influenced by cell size, endogenous esterase activity, etc. Using the inhibitors and calculating the MAF…
Tue, Jul 18, 2023
Inconsistent fluorescence shift using the same cell (irreproducible results).
Inadequate incubation condition. Always use a water bath (not incubator) and ensure temperature of water bath is 37°C.
Tue, Jul 18, 2023