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In Situ Hybridization and Detection Reagents
What is the size of ENZO PATHO-GENE® HPV probes and what regions do they target?
All of the PATHO-GENE® HPV probe we offer, vary in size from 100 bp to 1 Kb and the probe…
Tue, Jul 18, 2023
Does ENZO PATHO-GENE® HPV probes target E1, L2, E6 and E7 regions in the HPV genome? What is known about the targeting region of HPV probes?
All of the PATHO-GENE® HPV probes we offer, vary in size from 100 bp to 1 Kb and the probes…
Tue, Jul 18, 2023
How does PATHO-GENE® HPV Type 11 Probe, ENZ-GEN151 work?
The ENZO PATHO-GENE® HPV Type 11 Probe (ENZ-GEN151) is a biotin-labeled DNA probe designed to hybridize to HPV Type 11 DNA sequences. The…
Tue, Jul 18, 2023
Do you offer a CISH/FISH protocol?
Our ISH assay kits come with recommended manuals. However, we do not provide specific protocols for general ISH procedures as…
Tue, Jul 18, 2023
Can ISH probes also be used for Northern blots?
Yes, there is in principle no discriminating difference between probes for northern blotting or in situ hybridization of RNA targets.…
Tue, Jul 18, 2023
Can ISH be multiplexed with immunostaining?
Yes, both CISH can be combined with immunohistochemistry and FISH can be combined with immunofluorescence. However, multiplexing ISH with immunostaining…
Tue, Jul 18, 2023
Can BIO-PROBE®, PATHO-GENE®, or AMPIVIEW® ready-to-use ISH probes be used for FISH?
Biotin-labeled in situ probes are most commonly used for chromogenic detection, they can easily be adapted to fluorescent detection with…
Tue, Jul 18, 2023
How important are denaturation and hybridization temperature in ISH?
Correct denaturation and hybridization temperatures are paramount for successful ISH. Regardless of the probe or target being RNA or DNA,…
Tue, Jul 18, 2023
What are ISH probes typically labeled with?
Common labels include biotin, digoxigenin, and fluorochromes. Biotin is detected with streptavidin- conjugated enzymes followed by an enzymatic colorimetric reaction.…
Tue, Jul 18, 2023
Can multiple targets be detected with ISH?
Yes, it is common practice to stain with multiple ISH probes in parallel. However, while continuously-labeled immunofluorescent probes can be…
Tue, Jul 18, 2023