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Immunohistochemistry (IHC)
According to the instruction manual, HIGHDEF® red IHC chromogen (AP, plus) (ADI-950-141) produces a red color, ranging from pink to brilliant dark red. What does that depend on?
The color intensity depends on the levels of expression of the antigen of interest.
Tue, Jul 18, 2023
What is the difference between ADI-950-140 and ADI-950-141?
Some red alkaline phosphatase chromogens are unstable upon mixing. This can be problematic where an automated slide strainer is used. The…
Tue, Jul 18, 2023
Why is the SAVIEW® PLUS detection technology superior compared to the traditional ABC detection method?
The high affinity of the non-covalent interaction between biotin and streptavidin (Kd 4×10-14 M) forms the basis for our SAVIEW®…
Tue, Jul 18, 2023
Is the ISH/IHC Peroxidase block solution provided in the MULTIVIEW® PLUS IHC kit (ENZ-KIT181) the same as the IHC tissue primer (ADI-950-234)?
The peroxidase blocker solution is functionally the same as the IHC tissue primer but different in its formulation. The IHC tissue…
Tue, Jul 18, 2023
I plan to perform multiple IHC staining using your MULTIVIEW® PLUS IHC kit. What are your recommendations in case the targets co-localize?
In IHC, designing the experiment correctly is very critical. In the ideal situation, you will label unique proteins or antigens…
Tue, Jul 18, 2023
How can cross-reaction of detection reagents with primary antibodies be avoided using the MULTIVIEW® PLUS kit (ENZ-KIT181)?
Regarding the MULTIVIEW® PLUS (mouse-HRP/mouse-AP) IHC kit (Brown/Green), sufficient washes between antibody, detection, and chromogen steps (3 x 1 minute…
Tue, Jul 18, 2023
What is the difference between the mounting media ADI-950-260 and ADI-950-261?
HIGHDEF® IHC fluoromount (ADI-950-260) was specifically designed as an antifade reagent to cover tissues stained with fluorescent dyes. HIGHDEF® IHC…
Tue, Jul 18, 2023
How to prevent the formation of bubbles when using the HIGHDEF® IHC mount (ADI-950-261)?
In order to prevent bubble formation, please follow these recommendations:1) Remove red tip from the bottle and cut the tip off…
Tue, Jul 18, 2023