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Immunohistochemistry (IHC)
What is the lot-to-lot variation for CHO host cell protein ELISA kit, ENZ-KIT128?
In general, the antibodies provided with CHO host cell protein ELISA kit (ENZ-KIT12)8 are stable and we do not change…
Tue, Jul 18, 2023
What is the concentration of the PGE2-Alkaline phosphatase conjugate in the PGE2 ELISA kit, ADI-900-001?
Concentration of the PGE2-AP conjugate is proprietary and can't be revealed.
Tue, Jul 18, 2023
The chromogens are dissolving during dehydration, what may be the cause?
While most common chromogens are relatively resistant to organic solvents, some chromogens might be dissolved in xylene. Using xylene substitutes…
Tue, Jul 18, 2023
Why is peroxidase blocking necessary in colorimetric ISH/IHC detection?
When colorimetric detection by enzymatic reaction is used, a blocking step with peroxide solution is required to eliminate endogenous peroxidase…
Tue, Jul 18, 2023
Is protein blocking really necessary in ISH?
Blocking with bovine serum albumin (BSA), milk or serum is always recommended when ISH signals are detected by antibodies (e.g.…
Tue, Jul 18, 2023
How to cover up the sections during hybridization in ISH protocols?
The most common method is to cover tissue sections on glass slides in the hybridization solution with a glass cover…
Tue, Jul 18, 2023
Can I detect constitutive HSP70 with ADI-EKS-700B or ADI-EKS-715?
These assays are specific for induced HSP70 and will not detect constitutive HSP70. Taking this into account, you need to…
Tue, Jul 18, 2023
How much sample do I need to use and what are the suggested dilutions when using ADI-EKS-700B, ADI-EKS-715, ADI-EKS-725A, ADI-EKS-750, and ENZ-KIT-101?
The amount of sample required and the dilutions needed for your assay depend upon the amount of HSP70, HSP70B, or…
Tue, Jul 18, 2023
What are your recommendations as for mounting media and post-processing for your HIGHDEF® chromogens?
All our chromogens should be used preferentially with an aqueous-based mounting medium (e.g. ADI-950-261) for a better resolution and a…
Tue, Jul 18, 2023
Can I use your HIGHDEF® Chromogens with an alternative detection system (e.g. ABC)?
In theory, it is possible to use the HIGHDEF® Chromogens with an alternative detection system. It is recommended, however, to use our POLYVIEW® PLUS…
Tue, Jul 18, 2023