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Genomic Analysis
What is the purity, volume and amounts of Bio-17-ATP, ENZ-42817 provided?
Biotin-17-adenosine-5′-triphosphate is =93% (HPLC), the amount is 250 nmol in a volume of 25 ul.
Tue, Jul 18, 2023
What is the suggested volume of SEEBRIGHT® Red 598 dUTP, ENZ-42855 and SEEBRIGHT® Green 496 dUTP, ENZ-42831 with Nick translation DNA labeling system 2.0, ENZ-GEN111-0050?
We suggest to reconstitute the dyes (0.3 mM) and use the same volumes as mentioned in the manual of the…
Tue, Jul 18, 2023
Do we offer a kit to make cDNA from a low amount of RNA in the range 10-100 ng sample?
For cDNA preparation from low input RNA samples, we offer AMPIGENE® cDNA Synthesis Kit, ENZ-KIT106. The AMPIGENE® cDNA Synthesis Kit uses the…
Tue, Jul 18, 2023
What is the recommendation to reconstitute the SEEBRIGHT® lyophilized dUTPs, ENZ-42853L, ENZ-42843L, ENZ-42831L, ENZ-42842L?
Dissolving in 10 mM TE pH8, is the preferred method since the nucleotides are stable for long term -20°C storage.…
Tue, Jul 18, 2023
Can gene-specific primers be used for cDNA synthesis with BIOARRAY™ Single-round RNA amplification and biotin labeling system, ENZ-42420 and ENZ-42421?
RNA amplification with BIOARRAYTM Single-round RNA amplification and biotin labeling system requires the use of primers included in the kit.…
Tue, Jul 18, 2023
Does BIOARRAY™ Single-round RNA amplification and biotin labeling system, ENZ-42420 and ENZ-42421 have all the items necessary for amplification and labeling?
This kit, provided in 2 sizes (10 reactions: ENZ-42420; 100 reactions: ENZ-42421) contains all the buffers, enzymes, purification columns, elution…
Tue, Jul 18, 2023
Does AMPIPROBE® Bacterial Vaginosis Assay Kit, ENZ-GEN205-0100 include Megasphaera 1 as a control? Can this control be purchased as a stand alone item?
Positive PCR BV control in the AMPIPROBE® Bacterial Vaginosis Assay Kit (ENZ-GEN205) has Megasphaera as a kit component The extraction…
Tue, Jul 18, 2023
Can the dUTPs be used for TUNEL assays or only for ISH probe labeling?
The labeled dUTPs can be used for a variety of applications besides in situ hybridization. They can be used as…
Tue, Jul 18, 2023
What is the recommended ratio of dTTP to fluorophore-dUTP when using the Nick translation DNA labeling system 2.0?
The SEEBRIGHT® fluorophore-labeled dUTPs are supplied as a 1 mM solution. We recommend to prepare working concentration of 0.3 mM…
Tue, Jul 18, 2023
What are the correction factors for measuring dye incorporation in a NanoDrop?
The correction factors for 260/280nm detection are as follows: Correction Factor at Aqua 431 Green 496 Green 500 Gold 525 Gold 500 Orange…
Tue, Jul 18, 2023