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Enzyme Activity Assays
What is the recommended NAD+ concentration to be used when running BML-AK555?
This information can be found in the manual. Dilutions of NAD+ (BML-KI282, 50mM) in Assay Buffer (BML-KI286) have to be prepared…
Tue, Jul 18, 2023
Will EDTA or EGTA inhibit SIRT1 activity?
We have not specifically tested metal chelators on the sirtuins. Reaction inhibition by EDTA or EGTA is possible although inhibition…
Tue, Jul 18, 2023
Can you measure HDAC activity in cellular samples?
Yes, our FLUOR DE LYS® fluorometric activity assay kit (BML-AK500) has been developed to work with tissue/cell lysates, immunoprecipitates or…
Tue, Jul 18, 2023