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Enzyme Activity Assays
Why was my activation of SIRT1 by resveratrol not as strong as expected?
The kinetics of the reaction require some optimization. The optimal incubation time for the reaction can vary depending on concentrations…
Tue, Jul 18, 2023
Can I have further details about the HeLa nuclear extract included in your HDAC activity assay kits?
The HeLa nuclear extract is prepared by high salt extraction of HeLa nuclei using a modified protocol from the ones…
Tue, Jul 18, 2023
What is the enzyme composition of the HeLa nuclear extract included in your HDAC activity assay kits?
The HeLa nuclear extract is unpurified and therefore contains possibly all enzymes that can be found in the nucleus of…
Tue, Jul 18, 2023
When running an HDAC activity assay, what is the appropriate stock and working concentrations for my test compounds?
It is not possible for us to predict the appropriate stock solution or final assay concentrations for your test compounds…
Tue, Jul 18, 2023
Can I use the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit to measure SIRT1 activity in my cell/tissue extracts?
BML-AK555 is a drug discovery kit intended for screening potential activators or inhibitors of the recombinant SIRT1 provided in the…
Tue, Jul 18, 2023
What is the recommended NAD+ concentration to be used when running BML-AK555?
This information can be found in the manual. Dilutions of NAD+ (BML-KI282, 50mM) in Assay Buffer (BML-KI286) have to be prepared…
Tue, Jul 18, 2023
What is the amount or concentration of SIRT1 provided in BML-AK555?
The volume or concentration of SIRT1 is lot-dependent. Each kit contains 100 units of the enzyme. Concentration on specific lot of SIRT1 is available…
Tue, Jul 18, 2023
What are the excitation and emission spectra of the FLUOR DE LYS® substrate?
The fluorophore is excited with 360nm light and the emitted light is detected at 460nm on a fluorometric microplate reader.…
Tue, Jul 18, 2023
Can you measure the activity of SIRT1 with the FLUOR DE LYS® HDAC fluorometric activity assay kit?
This kit measures the activity of all HDACs provided with a biological sample. Simply changing the substrate from the FLUOR…
Tue, Jul 18, 2023
What is the recommended substrate for the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit?
The FLUOR DE LYS® SIRT1 substrate, the recommended substrate, is provided in the kit. The FLUOR DE LYS® SIRT1 substrate is a…
Tue, Jul 18, 2023