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Enzyme Activity Assays
Is the peptide substrate coated on the microtiter plate present in the ADI-EKS-390A kit specific for PKA?
No, the peptide substrate in the PKA kinase activity kit (ADI-EKS-390A) is not specific to PKA and therefore it can…
Tue, Jul 18, 2023
The kit insert indicates that the PKA enzyme is provided as positive control. Is it possible to use it to perform a standard curve?
The active PKA kinase provided in the kit is to be used as a positive control and is not intended…
Tue, Jul 18, 2023
I am seeing well-to well variability amongst triplicates in the standards and samples. Values are within expected range but varies. What could be the reason?
Well-to well variability might be due to less than optimal shaking. Make sure a gentle but thorough mixing is applied as indicated…
Tue, Jul 18, 2023
It appears that the positive control is not working properly. What could be the reason?
The nature of that protein is that it can quickly degrade unless it is handled very carefully. It must be…
Tue, Jul 18, 2023
What is the principle of the PKA kinase activity assay?
The PKA kinase activity kit allows relative quantification of PKA kinase activity in different sample types (purified, partially purified and…
Tue, Jul 18, 2023
Does the kit work for species other than human?
The ADI-EKS-390A kit is actually species independent; therefore, as long as there is sufficient PKA activity in your samples and…
Tue, Jul 18, 2023
What is the positive control provided in the kit?
The positive control provided in the kit ADI-EKS-390A is recombinant human PKAc beta (or catalytic subunit C-beta of PKA) expressed…
Tue, Jul 18, 2023
Can I use my lysis buffer instead of the lysis buffer indicated in the kit insert?
The buffer suggested in the kit insert has been optimized by our development scientists. It is likely that an alternative buffer will work,…
Tue, Jul 18, 2023
Can I use this kit to measure the concentration of PKA in my samples?
The kit does not measure the concentration of PKA in samples. It is intended for looking at relative PKA kinase activity.
Tue, Jul 18, 2023
Do I need a luminometer with double injectors for this assay?
Yes. A single injector will not provide good results. You cannot pre-mix the arachidonic acid and substrate to make a…
Tue, Jul 18, 2023