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Enzyme Activity Assays
Would COX activity assay kit, ADI-907-003 work with human plasma and mouse cell lysate samples?
COX activity assay kit, ADI-907-003 https://www.enzolifesciences.com/fileadmin/files/manual/ADI-907-003_insert.pdf is designed for the quantitative determination of COX activity in biological samples. However, we…
Tue, Jul 18, 2023
How specific are colorimetric/fluorogenic substrates in Matrix Metalloproteinase (MMP) drug discovery kits?
Several MMPs cleave both BML-P277-9090 substrate (OMNIMMP™ RED fluorogenic substrate peptide), BML-P278-9090 substrate (MPP-3 Fluorogenic Substrate peptide) and colorimetric substrate…
Tue, Jul 18, 2023
What is the formulation of BIOMOL® Green (BML-AK111)?
The formulation of BIOMOL® (BML-AK111) Green is proprietary.
Tue, Jul 18, 2023
I expect calcineurin to be mainly located in the nuclei. How should I treat my cell lysates in order to avoid loss of nuclei whe using Calcineurin cellular activity assay kit, BML-AK816?
You need to replace the high speed centrifugation suggested in the Calcineurin cellular activity assay kit (BML-AK816) manual which pellets…
Tue, Jul 18, 2023
Can the kit BML-AK804, Calcineurin Phosphatase assay kit be used for biological samples?
The product BML-AK804, Calcineurin Phosphatase assay kit is designed for inhibitor screening or kinetic assays using the recombinant Calcineurin enzyme…
Tue, Jul 18, 2023
The Calcineurin Phosphatase assay kit, BML-AK804, contains ½ volume microplate. What is the advantage of using this type of plate instead of a standard 96 well plate?
We use 1/2 volume plates for the Calcineurin Phosphatase assay kit (BML-AK804) because a longer path length improves accuracy for…
Tue, Jul 18, 2023
What is the recommend Calmodulin concentration to fully activate Calcinuerin in the Calcineurin Phosphatase assay kit, BML-AK804?
We provide 25µM Calmodulin with the Calcineurin Phosphatase assay kit (BML-AK804) and recommend a concentration of 0.5µM to fully activate…
Tue, Jul 18, 2023
Can the desalting resin provided with the kit BML-AK816, Calcineurin cellular activity assay kit be reused?
Yes, the desalting resin provided with the Calcineurin cellular activity assay kit (BML-AK816) can be reused for multiple samples. Between…
Tue, Jul 18, 2023
In the manual for the Calcineurin cellular activity assay kit, BML-AK816, you recommend using 5,000-50,000 cells per assay point. How much lysis buffer should be used to prepare the samples?
it is easier to dilute samples than to concentrate them. In order to prepare cells for use with Calcineurin cellular…
Tue, Jul 18, 2023
Could you recommend a control compound Calcineurin inhibitor to use with the Calcineurin Phosphatase assay kit, BML-AK804?
In the kit insert for our Calcineurin Phosphatase assay kit, BML-AK804, we list several inhibitors that were screened with the…
Tue, Jul 18, 2023