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CELLESTIAL® Assays
Do you offer different colored dyes with the same binding capacity to dsDNA?
We offer red, blue or green fluorescent dyes suitable for dsDNA binding, as a stand alone or as part of…
Tue, Jul 18, 2023
How long is the NUCLEAR-ID® red dye (ENZ-52406) retained by the cells and is the dye toxic to the cells?
NUCLEAR-ID® red DNA stain ( ENZ-52406) is fairly stable. We do not have specifics on how long the dye would be…
Tue, Jul 18, 2023
What is the reason for the use of dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020)? Should this be used with all cell lines or is there an exception?
The use of the dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020) can help preventing the…
Tue, Jul 18, 2023
How long is the CYTO-ID® long-term cell tracer dye (ENZ-51036, ENZ-51037) retained in the cell and what cells were used to validate the assay?
The dye is well retained by cells for up to 96-120 hours after loading depending on how fast the cells…
Tue, Jul 18, 2023
Is it possible to combine FLUOFORTE® Calcium Assay Kit (ENZ-51016) with other fluorescence dye in a multiplex assay? What is the fluorescence excitation and emission of the dye?
It is possible to multiplex FLUOFORTE® Calcium Assay Kit with other assays as long as there is no spectral overlap…
Tue, Jul 18, 2023
How specific is the NUCLEAR-ID® red dye (ENZ-52406) for dsDNA? What is the binding capacity of the dye to ssRNA, ssDNA and DNA:RNA Hybrids?
The dye stains dsDNA. Data is not available for binding capacity to ssRNA, ssDNA and DNA:RNA hybrids.
Tue, Jul 18, 2023
Is the dye efflux inhibitor (reagent B) in the FluoForte® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020), Probenecid ? Should this be added to every cell type to prevent the FluoForte from leaking out of the cells?
The inhibitor is similar in properties to Probenecid. Cells lacking active MDR pumps do not require addition of efflux inhibitor. Depending…
Tue, Jul 18, 2023
Is the Red NUCLEAR-ID® dye in the NUCLEAR-ID® Blue/Red cell viability reagent provided with reference ENZ-53005 the same dye as NUCLEAR-ID Red for cell cycle analysis provided with reference ENZ-51008?
No, these are two different dyes.
Tue, Jul 18, 2023
What is the recommended exposure setting for NUCLEAR-ID® red stain (ENZ-52406)?
The exposure depends on the microscope and filters. With a 50% neutral density filter, 1 second exposure is a good starting point.
Tue, Jul 18, 2023
Would diluting the sample to a lower concentration (e.g. 0.5 mg/mL) change the percentage of aggregate protein?
The percent aggregate (the ratio of aggregate to total protein) will stay the same. However, the concentration of aggregated protein…
Tue, Jul 18, 2023