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CELLESTIAL® Assays
How stable is the Cyto-ID® Green autophagy dye?
Fluorescent dyes are subject to photobleaching, but this dye is more robust than dyes like fluorescein. Standard handling precautions should…
Tue, Jul 18, 2023
Precipitate is seen in the 10X assay buffer
Precipitate forms at low temperatures. Allow solution to warm to room temperature or 37°C.
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® autophagy dye relative to monodansyl cadaverine (MDC)?
MDC requires 365 nm UV illumination and thus, is not compatible with 488 nm excitation sources commonly implemented in flow…
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® Autophagy Detection kit relative to GFP-LC3 transfected cells?
The most significant advantage for Cyto-ID® Autophagy Detection kit is that it's a no-transfection assay. Transfections are transient and there…
Tue, Jul 18, 2023