CELLESTIAL® Assays
Precipitate is seen in the 10X assay buffer
Precipitate forms at low temperatures. Allow solution to warm to room temperature or 37°C.
Tue, Jul 18, 2023
What is the detailed nature of the Cyto-ID® autophagy dye and how does the assay work?
The precise chemical structure of the dye is proprietary. The probe is a cationic amphiphilic tracer (CAT) dye that rapidly…
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® autophagy dye relative to monodansyl cadaverine (MDC)?
MDC requires 365 nm UV illumination and thus, is not compatible with 488 nm excitation sources commonly implemented in flow…
Tue, Jul 18, 2023
Are the cells fixable after staining with the autophagy kit?
Yes, a protocol for fixing cells is provided in the product manual. Permeabilization of the cellular membrane with detergents, however,…
Tue, Jul 18, 2023
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