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CELLESTIAL® Assays
How do the ROS/RNS dyes work?
Nitric Oxide (Red dye): The strategy for fluorescence-based detection of NO employs an o-phenylenediamine scaffold, which in the presence of…
Tue, Jul 18, 2023
For the ROS/RNS detection kit, ENZ-51001, can samples be stored after the final wash buffer step in the protocol, and do the microscopy at a later date?
No, this is a vital stain. Live cells must be read immediately per the manual.
Tue, Jul 18, 2023
Using this kit, is it necessary to wash the cells after staining?
No, the kit does not require a wash step.
Tue, Jul 18, 2023
How long can the fluorescence signal be detected in live cells?
Typically, cells are induced to undergo autophagy and then stained just prior to analysis. Induction can be as little as…
Tue, Jul 18, 2023
What is the Kd and quantum yield of FluoForte® Calcium Assay kit?
Kd is estimated to be 389 nM (22°C, pH 7.0 –7.5). Maximum quantum yield is 0.18 at pH 7.2.
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® Autophagy Detection kit relative to GFP-LC3 transfected cells?
The most significant advantage for Cyto-ID® Autophagy Detection kit is that it's a no-transfection assay. Transfections are transient and there…
Tue, Jul 18, 2023
Can the Cyto-ID® green detection dye be used in conjunction with another dye to differentiate between endosomes and amphisomes?
The Cyto-ID® Red long-term tracer dye, ENZ-51037- inserts into the plasma membrane and later is internalized by endocytosis. Cyto-ID® Red…
Tue, Jul 18, 2023
Are the cells fixable after staining with the autophagy kit?
Yes, a protocol for fixing cells is provided in the product manual. Permeabilization of the cellular membrane with detergents, however,…
Tue, Jul 18, 2023
Little or no apoptosis observed or detected
None or few apoptotic cells present in sample >> Use the Apoptosis Inducer, provided in the kit, as a positive…
Tue, Jul 18, 2023
Precipitate is seen in the 10X assay buffer
Precipitate forms at low temperatures. Allow solution to warm to room temperature or 37°C.
Tue, Jul 18, 2023