
Does Cellular senescence activity assay, ENZ-KIT129 require running standards and are standards provided with this kit?
Cellular senescence activity assay, ENZ-KIT129 is designed as semi quantitative assay. Therefore, standards are not provided. As shown on page 7…
Tue, Jul 18, 2023

Are the reagents in MITO-ID® Membrane potential detection kit, ENZ-51018 sterile? Can the reagents in this kit be used in cell culture and flow cytometry application?
Reagents in MITO-ID® Membrane potential detection kit, ENZ-51018 are not sterile. The reagents in this kit are optimized for measurement of…
Tue, Jul 18, 2023

Why do we recommend 2,500 fold dilution of MITO-ID® Red Detection reagent, ENZ-51007 for staining aldehyde fixed and detergent permeabilized cells, but 10,000 fold dilution of MITO-ID® Detection reagent for live cells?
In aldehyde-fixed and detergent permeabilized sample, MITO-ID® Red Detection reagent diffuses passively once the membrane potential is lost. Therefore, higher dye…
Tue, Jul 18, 2023

Can test compound dissolved in DMSO be used in cell based assay to measure Reactive Oxygen Species with ROS-ID® Total ROS/Superoxide detection kit, ENZ-51010
We cannot confirm if dissolving the compound in DMSO could affect the assay, since DMSO could inhibit hydroxyl radical generation…
Tue, Jul 18, 2023

Can cells be fixed with 4% paraformaldehyde with the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175)?
A fixation step may be included when using the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175), but it needs to…
Tue, Jul 18, 2023

Is Autophagy Detection reagent in ENZ-51031 and ENZ-KIT175 compatible with anti-fluorescence quenching agent and fluoromount-G?
The CYTO-ID® Autophagy Detection reagent in ENZ-51031 and ENZ-KIT175 kits is compatible with anti-fluorescence quenching agent and Fluoromount-G.
Tue, Jul 18, 2023

Can the microplate be read following addition of ROS/Superoxide Detection Solution with ROS-ID® Total ROS/Superoxide detection kit, ENZ-51010?
Yes, plates can be read using bottom reading option without removing the ROS/Superoxide Detection Solution and without washing.
Tue, Jul 18, 2023

What is the difference between Hoechst 3342, ENZ-52401 and Hoechst 33258, ENZ-52402?
Hoechst 33258 (ENZ-52402) is less cell permeable than other derivatives, however it also allows for quantitative measurements when plotted in…
Tue, Jul 18, 2023

How does MITO-ID® dye in the Membrane Potential kits, ENZ-51018 and ENZ-51019 detect membrane potential?
MITO-ID® Membrane Potential Kits (ENZ-51018, ENZ-51019) measures mitochondrial membrane potential with a cationic dye dual-emission dye that exists as green…
Tue, Jul 18, 2023

What advantages does MITO-ID® Membrane Potential Kits, ENZ-51018 and ENZ-51019 offer compared to JC-1 and JC-10?
Compared with the commonly used cationic carbocyanine dye JC-1 and JC-10, the MITO-ID® membrane potential dye (ENZ-51018 and ENZ-51019) offers…
Tue, Jul 18, 2023