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Cell Cycle Analysis
Chromatin condensation is not observed
Incorrect drug concentration or incubation time for cell type: Make sure the cell preparation protocol generates single cells with minimal…
Tue, Jul 18, 2023
More than two populations appear upon apoptosis induction.
It's the property of the dye. Apoptosis is a multi-stage process and Nuclear-ID green dye can distinguish different levels of…
Tue, Jul 18, 2023
Healthy cells show very high fluorescence intensity
PMT setting is too high. Lower PMT setting on flow cytometer. Incubation temperature. Incubation with the dye should be at…
Tue, Jul 18, 2023
DNA histograms are of poor overall quality.
The key elements in obtaining a histogram of high quality are good sample preparation, correct cell-to dye ratio and proper…
Tue, Jul 18, 2023
What is actually being stained in the nucleolus by the Nucleolar-ID™ Green Detection Reagent?
We use an RNA binding dye and this has been validated. However, in the context of live cells we cannot…
Tue, Jul 18, 2023
Using this kit, is it necessary to wash the cells after staining?
No, the kit does not require a wash step.
Tue, Jul 18, 2023