AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Calcium Mobilization
Can you provide an overview of steps in Intracellular Calcium mobilization assay with FLUOFORTE® Calcium assay kit, ENZ-51016 ?
The main steps for the FLUOFORTE® Calcium assay kit (ENZ51016) involves loading cells with dye that fluoresce when exposed to…
Tue, Jul 18, 2023
What is the structure of the FluoForte dye and how does the dye detect Calcium levels in the cell?
The fluorogenic calcium-binding dye is a proprietary acetoxymethyl (AM) ester, which is cell-permeable. Once the dye enters the cells, it…
Tue, Jul 18, 2023
Is the dye efflux inhibitor (reagent B) in the FluoForte® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020), Probenecid ? Should this be added to every cell type to prevent the FluoForte from leaking out of the cells?
The inhibitor is similar in properties to Probenecid. Cells lacking active MDR pumps do not require addition of efflux inhibitor. Depending…
Tue, Jul 18, 2023
Is it possible to detect changes in calcium level in cells nuclei following specific treatment with the FLUOFORTE® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020)?
Detecting the calcium level in cells nuclei would require optimization. FLUOFORTE® Calcium Assay Kits (ENZ-51016, ENZ-51017, ENZ-51020) have been benchmarked for…
Tue, Jul 18, 2023
Is it possible to combine FLUOFORTE® Calcium Assay Kit (ENZ-51016) with other fluorescence dye in a multiplex assay? What is the fluorescence excitation and emission of the dye?
It is possible to multiplex FLUOFORTE® Calcium Assay Kit with other assays as long as there is no spectral overlap…
Tue, Jul 18, 2023
Can the FluoForte® Calcium assay kit be used to monitor Ca2+ flux in nicotinic acetylcholine receptors on Flexstation. Can the FluoForte dye monitor Ca2+ in our model system given that FluoForte dye has been validated only in GPCR signaling path
While no specific references are available for application of FluoForte to assay nicotinic receptor, there are numerous publications regarding the…
Tue, Jul 18, 2023
Can calcium mobilization be observed over the course of 1 hour with FluoForte Calcium assay kit? How long can the cells retain the fluorescent dye?
Ligand binding to GPCR generates very fast kinetics for calcium release, with the mobilization complete in less than a minute…
Tue, Jul 18, 2023
Fluorescence drop upon compound addition
Cells may become dislodged during addition. Decrease the rate of addition or seed fewer cells per well.
Tue, Jul 18, 2023
Untreated cells have calcium response
Inconsistent DMSO concentration. Ensure that buffer used for the negative control wells has the same final concentration of DMSO as…
Tue, Jul 18, 2023
High baseline fluorescence
Growth medium and organic anion transport contribute to high baseline fluorescence. Remove the medium before adding indicator dye to the wells.…
Tue, Jul 18, 2023