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Aggregate Detection
What thermal cyclers have the protocol been tested with ProteoStat® Thermal shift stability assay kit, ENZ-51027?
ProteoStat® Thermal shift stability assay should work on all types of thermal cyclers in the market. It has been tested…
Tue, Jul 18, 2023
What detergents are compatible with ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027 and ProteoStat® Protein Aggregation Assay?
The ProteoStat® dye is compatible with some detergents, such as 0.1% Tween 20, but this varies with the protein used. …
Tue, Jul 18, 2023
What are the differences between ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, Protein Aggregation Assay kit, ENZ-51023, and ProteoStat® Aggresome Detection kit, ENZ-51035?
ProteoStat® dyes belongs to the class of molecular rotor dyes. They are non-fluorescent in solution due to free rotation around…
Tue, Jul 18, 2023
Can the ProteoStat® TS Detection Reagent in the thermal shift stability assay kit, ENZ-51027, be substituted with the detection reagent from the ProteoStat® Protein Aggregation Assay, ENZ-51023?
Theoretically, the two dye reagents can be interchanged but this would require some optimization.
Tue, Jul 18, 2023
Would the aggregation temperature as determined using ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, be the same as determined using the Thermofluor technique?
In Thermofluor technique, SYPRO Orange, an environment-sensitive dye, is used to measure the unfolding of the protein as the temperature…
Tue, Jul 18, 2023
Is the binding of the protein to the ProteoStat® detection dye reversible?
The interaction between the ProteoStat® dye and the protein aggregates is non-covalent. So, in theory it is a reversible interaction.…
Tue, Jul 18, 2023
Is it possible to distinguish between protein aggregates of different sizes using ProteoStat® Protein Aggregation Assay, ENZ-51023?
The ProteoStat® detection dye does not differentiate aggregate sizes. The dye will bind to a wide range of protein aggregate…
Tue, Jul 18, 2023
What are the applications for ProteoStat® Thermal shift stability assay kit, ENZ-51027?
The ProteoStat® Thermal Shift Stability Assay kit (ENZ-51027) is used for direct monitoring of protein aggregation arising from a thermally-induced…
Tue, Jul 18, 2023
How different is ProteoStat® TS Detection Reagent in ENZ-51027 from ProteoStat® Detection Reagent in ENZ-51023?
ProteoStat®TS Detection Reagent in ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, and ProteoStat®Detection Reagentin ProteoStat® Protein Aggregation Assay, ENZ-51023, are…
Tue, Jul 18, 2023
What is the recommended storage condition for ß-Lactoglobulin solution?
The solution of ß-Lactoglobulin can be stored at 4°C for up to a month. For long term storage, it is…
Tue, Jul 18, 2023