Why do we recommend 2,500 fold dilution of MITO-ID® Red Detection reagent, ENZ-51007 for staining aldehyde fixed and detergent permeabilized cells, but 10,000 fold dilution of MITO-ID® Detection reagent for live cells?

In aldehyde-fixed and detergent permeabilized sample, MITO-ID® Red Detection reagent diffuses passively once the membrane potential is lost. Therefore, higher dye concentration enables better visualization as the dye begins to leach and the intensity is lost with time.

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