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There are two aspects making Aqua-labeled probes harder to detect: First, the aqua fluorophore (DEAC – 7-Diethylaminocoumarin-3-carboxylic acid) has a comparably low extinction coefficient. While it offers stronger signal than most other blue fluorophores compared to other colors, Aqua will absorb less of the available light resulting in weaker signal. Second, the emission spectrum of the Aqua dye falls into a part of the light spectrum to which the human eye is relatively insensitive. Digital imaging or confocal fluorescence microscopy is therefore much better suited to detect the Aqua dye than direct microscopy and detection by eye. Additionally, digital signal acquisition will allow to boost the signal by exposure time and signal gain.