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When preparaing an assay with Tyrosinase (human), (recombinant) (His-tag), BML-SE535, please consider that Substrate-1, MBTH (3-methyl-2-benzothiazolinone hydrazone) is unstable. Make 20mM stock fresh and keep sealed in the dark until use.
Substrate-2,L-Tyrosine needs to be dissolved in 1M HCl. Make 50mM (9mg/ml) stock in 1M HCl. Store at -20C and avoid freeze/thaw cycles.