We suggest to reconstitute the dyes (0.3 mM) and use the same volumes as mentioned in the manual of the Nick translation DNA labeling system 2.0 (ENZ-GEN111). A ratio of 1:2 volume of fluorophore-dUTP:dTTP is typically sueggested for both SEEBRIGHT® Red 598 dUTP (ENZ-42855) and SEEBRIGHT® Green 496 dUTP (ENZ-42831).