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Have You Published Using an Enzo Product?
The excitation and emission spectra of the dye is provided on page 12 of the kit manual. The spectrum indicates a slight overlap with FITC. However, FITC has been successfully used with this dye, using a 488nm excitation filter and a 515nm emission filter with narrow cutoff. This works if the FITC is bright. Otherwise, there will be some spillover from the ProteoStat dye. Setting up a control without the FITC-conjugated antibody would give an idea of the amount of spectral bleed through from Proteostat. As an alternative to FITC, antibody labeled with Cy5 or maybe Coumarin would address the issue.