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Ideal section thickness depends on fixation and embedding method, as well as microscope that is available. FFPE tissue is ideally cut to 3-4 µm thickness, while cryopreserved tissue is often cut to 5-8 µm sections. Too thin sections are not only a challenge to handle, but can result in truncated signal. Too thick sections might cause issues with hybridization efficiency and may lead to overlapping signals. While confocal microscopy helps to overcome the latter easily and can tolerate thicker sections, epifluorescence or transmission light microscopes require thin sections.