What is the recommendation for dye to cell ratio for microscopy and flow cytometry staining with the MITO-ID® Membrane potential detection kit, ENZ-51018?

For detailed information on the protocol, please follow the indications provided in the manual avalable from download on MITO-ID® Membrane potential detection kit, ENZ-51018 webpage: https://www.enzolifesciences.com/ENZ-51018/mito-id-membrane-potential-detection-kit/. As indicated in this document, adherent cells should be cultured until ~80% confluent. Suspension cells should be cultured to a density not to exceed 1 x 10⁶ cells/ml. The Dual Detection Reagent can be prepared by adding 10 µl of MITO-ID MP Detection Reagent and 4 µl of Necrosis Detection Reagent to every 1 ml of 1x Assay Solution.

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