AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
The use of the dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020) can help preventing the dye to be pumped out of the cell. More specifically, once inside the cells, the FLUOFORTE® dye is hydrolyzed by intracellular esterases, which leads to the generation of a membrane-impermeable form. However, some cells might have multi-drug resistance (MDR) transporters able to pump the dye out, thus lowering the signal. This can be prevented by adding the dye efflux inhibitor included in the kit. Nonetheless, it is important to consider that the choice of the inhibitor can depend on which MDR transporter are expressed the cells you are working with. Depending on the cell line, Verapamil, MK-571 or Novobiocin, can be added to inhibit specific MDR pumps. Cells lacking active MDR pumps do not require addition of efflux inhibitor. Literature data can help in the definition of the best experimental conditions for each specific cell type.