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This is the minimum dilution required to eliminate matrix interference in the assay. In general, this data is collected using a pool of samples from each matrix and evaluated using linearity, spike & recovery, and parallelism. A larger dilution may be necessary for your specific samples. You should perform your own validation experiments to determine the optimal dilution for your specific samples. However, we do not recommend diluting less without doing the tests. If you have low levels of analyte in your sample, extraction is a better option for you to return accurate sample values.