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The Green detection reagent included in the kit ENZ-51028 is a fluorescent sphingolipid analog that is metabolized by cells and transported directly into the Golgi bodies where-upon it interacts with endogenous lipids of that organelle. The dye is restricted to the luminal leaflet of the Golgi body membrane. The fluorescent lipid appears to preferentially stain the trans-Golgi stacks of the organelle.