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LYSO-ID® detection dyes provided with references ENZ-51034, ENZ-51015, ENZ-51005 are proprietary cationic amphiphilic tracers (CAT) that rapidly and selectively stains acidic organelles, and are therefore suitable for monitoring accumulation of lysosomes and lysosome-like structures in live cells. The dye has titratable groups to enable labeling of lamellar inclusion bodies of cells pretreated with weakly basic cell-permeable compounds, such as the antimalarial drug chloroquine. Besides lysosomes, the probe can be employed for highlighting lysosome-like organelles under conditions wherein cells produce vacuoles that contain hydrolytic enzymes but are not as acidic as the parent organelle.