What is the likely explanation for lack of staining cells with NUCLEAR-ID® Red DNA stain, ENZ-52406 for FACS application, when 250-fold and 500-fold dilution and incubation at 37°C for 30 minutes were tried?

 It is important to check cell density and to ensure that cell concentration is 1 x 10⁵ to 5 x 10 ⁵ cells/ml. It is important to achieve a monodisperse cell suspension at this step by gently pipetting up and down
repeatedly. A longer incubation with dye might help improve or sharpen the signal. Counting more cells would be helpful if possible. Do not hesitate to reach out to Enzo tech support to further discuss your data.

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