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Concerning the components of the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, the hypoxia detection reagent is relatively stable and can be stored at -20°C for one year. The lyophilized oxidative stress reagent is relatively stable in absolutely dry conditions stored at -20°C. Upon reconstitution in anhydrous DMP, the stock solution should be stored at -20°C. The shelf life of the reconstituted reagent is about 1 week at -20°C, because of the nature of the dye. It might be possible to slightly increase the stability to a few weeks if storing the probe at -80°C. Another possibility would be to reconstitute the probe in ethanol 100% or DMF, prepare aliquots of the needed volume and re-lyophilize them immediately (for example using a SpeedVac). The dry aliquots can then be stored at -80°C. However, it is important to highlight that we do not have exact information concerning the stability of the probe in these conditions.