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Cell or tissue lysis can be enhanced by adding 0.1-1% Triton X-100 to 0.1M HCl prior to use. When used in this concentration range, the detergent will not interfere with acetylation or the binding portion of the assay. However, there will be a modest increase in the optical density. Samples containing Triton X-100 should be evaluated against a standard curve diluted in the same Triton X-100 containing buffer for the most accurate determination.