What are the main advantages of working with ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027?

The ProteoStat® Thermal Shift Stability Assay kit works over a wide temperature, pH and ionic strength range and is compatible with commonly used buffers and excipients. Fluorescent dye-based screening methods for protein aggregate detection offer a time-saving approach with increased throughput and low material consumption relative to conventional instrumentation-intensive analytical methods.

Nile Red, Sypro Orange and 1,8-ANS detect protein unfolding through dye interaction with newly exposed hydrophobic surfaces. They are sensitive to the hydrophobicity of the surrounding environment in which they are employed, and as such, these environmentally sensitive dyes tend to be less specific when detecting protein aggregation and are susceptible to hydrophobic compound interference.