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MDC requires 365 nm UV illumination and thus, is not compatible with 488 nm excitation sources commonly implemented in flow cytometry. The Cyto-ID® Autophagy Detection kit employs a 488 nm-excitable green-emitting fluorescent probe to highlight the various vacuolar components of the autophagy pathway. It should be noted that unlike the lysomotrophic dyes; MDC, LysoTracker™ Red and Acridine Orange, which primarily detect lysosomes, the Cyto-ID® autophagy dye only very weakly stains lysosomes, while serving both as a selective marker of autolysosomes and earlier autophagic compartments