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Limit of detection is a statistical measurement meant to indicate the lowest concentration that the assay can differentiate from zero more specifically at 2 standard deviations (SD) above background. It is calculated from a high number of replicate 0 samples. This number can be smaller than the lowest point on the standard curve. You can use the sensitivity of the kit as the limit of what you can detect. However, sample values read between 2SD and the lower standard in the kit can only be reported as higher than the limit of detection of the assay and the data might not be accurate. It is always recommended to analyze data that fall in the dynamic range of the assay for accuracy and reproducibility.