The datasheet for my compound states to add BSA or serum to the aqueoussolution before attempting to dilute my stock solution. Why is this suggested?

Date Published: July 18, 2023

BSA and other serum albumins are natural carrier proteins for hydrophobic small molecules such as hormones and fatty acids. They are highly water soluble yet contain distinct hydrophobic binding domains that bind non-specifically to many hydrophobic molecules. The association of a hydrophobic small molecule with BSA can often help to keep the hydrophobic small molecule in solution.
In general, BSA will form a 1:1 association with a hydrophobic small molecule, so the molar concentration of the BSA should be the same as, or slightly higher than the molar concentration you are attempting to achieve with the small molecule. As with the organic chemical solvents, it is important to carry out appropriate solvent controls with your experiments.

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