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What is the enzyme composition of the HeLa nuclear extract included in your HDAC activity assay kits?
The HeLa nuclear extract is unpurified and therefore contains possibly all enzymes that can be found in the nucleus of…
Tue, Jul 18, 2023
Can you measure the activity of SIRT1 with the FLUOR DE LYS® HDAC fluorometric activity assay kit?
This kit measures the activity of all HDACs provided with a biological sample. Simply changing the substrate from the FLUOR…
Tue, Jul 18, 2023
Can I use the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit to measure SIRT1 activity in my cell/tissue extracts?
BML-AK555 is a drug discovery kit intended for screening potential activators or inhibitors of the recombinant SIRT1 provided in the…
Tue, Jul 18, 2023
What is the recommended NAD+ concentration to be used when running BML-AK555?
This information can be found in the manual. Dilutions of NAD+ (BML-KI282, 50mM) in Assay Buffer (BML-KI286) have to be prepared…
Tue, Jul 18, 2023
How should KillerTRAIL™ and SuperKillerTRAIL™ be handled and stored to best assure stability of their biological activity?
Once thawed, it is recommended that appropriate aliquots are prepared and stored at -80°C. In order to retain full activity,…
Tue, Jul 18, 2023
Will EDTA or EGTA inhibit SIRT1 activity?
We have not specifically tested metal chelators on the sirtuins. Reaction inhibition by EDTA or EGTA is possible although inhibition…
Tue, Jul 18, 2023
Can you measure HDAC activity in cellular samples?
Yes, our FLUOR DE LYS® fluorometric activity assay kit (BML-AK500) has been developed to work with tissue/cell lysates, immunoprecipitates or…
Tue, Jul 18, 2023