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What are the MW of KillerTRAIL™ and SuperKillerTRAIL™?
Enzo Life Sciences’ KillerTRAIL™ and SuperKillerTRAIL™ monomers have a calculated MW of 24,144 Da and 24,350 Da, respectively. Under reducing…
Tue, Jul 18, 2023
Can I use the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit to measure SIRT1 activity in my cell/tissue extracts?
BML-AK555 is a drug discovery kit intended for screening potential activators or inhibitors of the recombinant SIRT1 provided in the…
Tue, Jul 18, 2023
What is the recommended substrate for the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit?
The FLUOR DE LYS® SIRT1 substrate, the recommended substrate, is provided in the kit. The FLUOR DE LYS® SIRT1 substrate is a…
Tue, Jul 18, 2023
What is the recommended NAD+ concentration to be used when running BML-AK555?
This information can be found in the manual. Dilutions of NAD+ (BML-KI282, 50mM) in Assay Buffer (BML-KI286) have to be prepared…
Tue, Jul 18, 2023
When running an HDAC activity assay, what is the appropriate stock and working concentrations for my test compounds?
It is not possible for us to predict the appropriate stock solution or final assay concentrations for your test compounds…
Tue, Jul 18, 2023
Will EDTA or EGTA inhibit SIRT1 activity?
We have not specifically tested metal chelators on the sirtuins. Reaction inhibition by EDTA or EGTA is possible although inhibition…
Tue, Jul 18, 2023
Can you measure HDAC activity in cellular samples?
Yes, our FLUOR DE LYS® fluorometric activity assay kit (BML-AK500) has been developed to work with tissue/cell lysates, immunoprecipitates or…
Tue, Jul 18, 2023