Non-Antibody Based Assays

Activity Assays
Activity assays do not use antibodies as detection reagents. The kits measure the activity of enzymes by measuring the amount of a specific substrate that is turned-over by the enzyme being analyzed. Many activity assays have homogeneous mix-and-read formats that do not require wash steps. They are often adaptable to multiple assay formats including kinetic, end-point, and inhibition assays.

Detection Assays
As with activity assays, detection assays do not use antibodies as detection reagents. Very small molecular weight analytes require relatively simple biochemical techniques to measure their concentrations. Nitric Oxide and Hydrogen Peroxide assays are examples of detection kits for very small molecular weight antigens. In these kits, chemically driven color changes are used to measure the concentration of these molecules in samples. Detection assays also have simple homogeneous mix-and-read format.

Acridinium Ester
We supplie a Chemiluminescent Labeling Kit for use with antibodies, proteins, peptides, and nucleic acids. Acridinium ester-labeled samples can be used for a number of different applications. They can be added as tracers to samples containing unlabeled materials as an alternative to the use of 125I labeled molecules. They can also be used as labeled antigens for competitive immunoassays or as labeled signal antibodies for immunometric assays. In addition, chemiluminescent-labeled nucleic acids may be used as reporter molecules in homogeneous DNA probe-based systems.