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Methanol fixation (for cytoskeletal components)
The methanol fixation is an easy method. However, it frequently solubilizes and removes membrane bound antigens. By a simple precipitation of the protein, methanol only provides low structural preservation.
- Rinse the cover glass with PBS.
- Fix cells by incubating the cells in pre-cooled 100% methanol at -20°C for 10 minutes.
- Wash cells with PBS.