It says in the insert that samples containing high levels of NADPH need to be diluted sufficiently. How can I determine the NADPH levels and dilution prior to running the Nitric Oxide detection assay?

We have found that 0.5-1mM NADPH will inhibit the Griess color reaction slightly. If this amount of NADPH is present the sample ODs can be artificially but modestly depressed. In order to determine the dilution necessary, a serial dilution of a spiked sample can be run. If there is no interference, the recoveries will be linear.

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